RNAi Screening Libraries

siRNA Screening Libraries

• Cherry-pick libraries
  Freedom with custom library design. Fast and easy online configuration and ordering of plated siRNA & microRNA reagents.

• Human siRNA libraries
  Choose from predefined collections of gene families, druggable targets, and the whole genome.

• Mouse siRNA libraries
  Choose from predefined collections of gene families and druggable targets.

shRNA Screening Libraries

• Cherry-pick clone libraries
  Choose from our predesigned product lines of shRNA and over-expression reagents to build your own custom library.

• SMARTvector lentiviral shRNA pooled libraries
  The most advanced shRNA screening tool available. Achieve highly effective gene knockdown using our optimized scaffold combined with promoter and reporter flexibility.

• GIPZ lentiviral shRNA libraries
  Perform genome-scale shRNA screening experiments with GIPZ Lentiviral shRNA libraries.

• TRIPZ inducible lentiviral shRNA libraries
  Human genome-wide collection available, with an inducible promoter for the study of essential gene function.

• TRC lentiviral shRNA libraries
  Human and mouse genome-wide collections available as glycerol stocks.

• Pooled lentiviral screening libraries
  Powerful multiplexed screening tools to investigate thousands of genes with a single transduction event.

• SMARTvector inducible lentiviral shRNA pooled libraries
  Choose the best promoter and reporter for the optimal experiment. Combines the advantages of pooled shRNA screening with the power of an inducible promoter.

microRNA Screening Libraries

• Cherry-pick libraries
  Fast and easy online configuration and ordering of plated siRNA & microRNA reagents targeting your genes of interest.

• miRIDIAN microRNA libraries
  Mimic and hairpin inhibitor libraries available for human, mouse, and rat microRNAs. Provided in 96-well plates for high-throughput phenotypic screening.

• Pooled lentiviral screening libraries
  Investigate thousands of genes with a single transduction event with powerful multiplexed screening tools.

Custom Screening Libraries

• Cherry-pick libraries
  Efficient online configuration and ordering of plated siRNA & microRNA reagents targeting your genes of interest.

• Cherry-pick clone libraries
  Choose from our predesigned product lines of shRNA and over-expression reagents to build your own custom library.

Bacterial glycerol stock
Bacterial culture transformed with a plasmid vector, grown in the presence of glycerol for freezer stock.

SMARTpool
A mixture of four siRNAs provided as a single reagent. siGENOME & ON-TARGETplus 2.0 siRNA SMARTpool reagents are guaranteed* to silence by 75% or better.

Four unique siRNAs targeting a single gene provided as individual reagents in an array of four. Three of four siGENOME and ON-TARGETplus 2.0 siRNAs are guaranteed* to silence by 75% or better.

Transduction-ready high-titer Lentiviral particle format.

Gene families and pathways
Predefined libraries arranged by gene family and/or function.

Arrayed plates
Arrayed in 96-well or 384-well microtiter plates. ECHO acoustic dispensing plates available on request.

ON-TARGETplus 2.0 and siGenome siRNA reagents (SMARTpool and three out of four individual siRNAs) are guaranteed to silence target gene expression by at least 75% at the mRNA level compared to non-targeting controls as measured by qPCR when demonstrated to have been used under optimal delivery conditions (confirmed using functionally validated positive control and measured at 24 to 72 hours after transfection using 100 nM siRNA).

Note: While we guarantee knockdown >75% at 100 nM, most ON-TARGETplus 2.0 and siGenome siRNA products are highly functional at 5-25 nM working concentration. Titration is recommended to maximize on-target siRNA effect and minimize any off-target behavior. Optimal siRNA delivery conditions and working concentration are experiment specific.

Please reach out to our technical support team for any questions or for guidance on optimizing the use of your siRNA products.

Which RNAi screening library suits your experimental needs?

Use the table below to assist you in determining the right RNAi screening library for your needs.

	Predefined siRNA libraries	Predefined shRNA libraries	SMARTvector Lentiviral shRNA libraries	miRIDIAN Mimic and Hairpin Inhibitor libraries
Species	Human, Mouse	Human, Mouse	Human, Mouse, Rat	Human, Mouse, Rat
Available as genome-scale collections
Available as custom library
Formats	Gene families Arrayed plates SMARTpool Set of four	Gene families Arrayed plates Glycerol Stock	Gene families Lentiviral particles	Arrayed plates